Answer:
false
Explanation:
Polymerase chain reaction is used to amplify a DNA segment. This reaction completes in 3 steps that are Denaturation, annealing, and extension.
Denaturation: The DNA segment is separated into the single strand by applying high temperature up to 95°c for 30 seconds.
Annealing: During annealing temperature gets lower and the primer gets anneal on the DNA templates.
Extention: During extension temperature is again increased up to 72°c and Taq polymerase is used to add nucleotide in the growing chain.
Therefore Taq polymerase does not separate DNA strands during annealing step rather it extends the new strand of DNA in extension step.
